Approaches
Circadian behavioral analysis
(wheel running, infrared tracking, feeding)
Neuromodulation
(in vivo, in vitro optogenetics, chemogenetics)
- Circadian neurons in the paraventricular nucleus entrain and sustain daily rhythms in glucocorticoids
- SCN VIP neurons are essential for normal light-mediated resetting of the circadian system
- Entrainment of circadian rhythms depends on firing rates and neuropeptide release of VIP SCN neurons
- Manipulating circadian clock neuron firing rate resets molecular circadian rhythms and behavior
In vivo imaging
(fiber photometry recording of calcium and clock genes)
In vitro imaging
(time-lapse recording of calcium and clock genes)
- Circadian neurons in the paraventricular nucleus entrain and sustain daily rhythms in glucocorticoids
- SCN VIP neurons are essential for normal light-mediated resetting of the circadian system
- Entrainment of circadian rhythms depends on firing rates and neuropeptide release of VIP SCN neurons
- The core clock gene Per1 phases molecular and electrical circadian rhythms in SCN neurons
- Manipulating circadian clock neuron firing rate resets molecular circadian rhythms and behavior
Neurogenetics
(CRISPR/Cas9 editing of clock genes and receptors)
Neuroanatomy
(circuit tracing, IHC, confocal imaging)
Manipulation and measurement of hormones
(ELISA, drug delivery, gonadectomy)
Complex behavioral analysis
(video tracking, machine learning)
Open-source tool development
(3D printing, laser cutting, electronics)